Chloride is a critical component of all living cells.
Voltage-gated chloride channels regulate cellular traffic of chloride ion.
The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport.
Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis.
In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb).
Additional forms of these proteins are obtained by alternative splicing.
All CLC proteins (~700-1000 aa) are predicted to contain 10 (possibly 12) transmembrane domains.Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues.
Rat CLC-6 is 803 aa membrane protein (human CLC-6 869 aa; mouse 870 aa) (1).
Alternative splicing of CLC-6 generates 4 isoforms, CLC-6a-6d (2).
CLC-6a is the full length form, while forms 6b-6d are truncated at their C-terminus and they contain 4 TM (CLC-6b and CLC-6d) or 7 TM (form CLC-6c).
CLC-6a and CLC-6c are the most abundant isoforms with CIC-6a being the most abundant isoform with CLC-6a being ubiquitously expressed and CLC-6c being limited to kidney (1, 2).
CLC-6 also is expressed in testis, ovary, small intestine, brain, and skeletal muscle.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 40% glycerol.
Specificity：Recognizes rat CLC6. No significant sequence homology is detected with other CLCs or other proteins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo Rt
Immunogen：A 19aa peptide sequence near the C-terminus of rat CLC-6 (KLH). Species sequence homology: mouse and human.
