Chloride is a critical component of all living cells.
Voltage-gated chloride channels regulate cellular traffic of chloride ion.
The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport.
Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis.
In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb).
Additional forms of these proteins are obtained by alternative splicing.
All CLC proteins (~700-1000 aa) are predicted to contain 10 (possibly 12) transmembrane domains.Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues.
Rat CLC-K1 is 687 aa membrane protein (human 687 aa) (1).
CLC-K1 is ~84% identical with CLC-K2 (2).
CLC-K1 is specific for kidney (cortical thick ascending limb and the distal convoluted tubule with minor expression in the S3 segment of the proximal tubule and the cortical collecting tubule).
It may play a role in urine concentrating mechanism.
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Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
Antibodies made to CLC-K1 C-terminal regions has detected proteins of ~70kD in kidney (2)
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified over the peptide-Sepharose column
Form：Supplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 0.09% sodium azide, 40% glycerol.
Specificity：The 17 AA rat control peptide is 93% conserved in human CLC-K1 or CLC-Ka. It also has high homology with rat CLC-K2 and 81% with human CLC-K2. Anti-rat CLCK11 should crossreact with both CLC-K1 and CLC-K2. No significant sequence homology is detected with other CLCs or other proteins. Actual crossreactivity of antibodies in various species has not been studied. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：A 17 AA Peptide sequence near the C-terminus of rat CLC-K1 (1). The peptide was coupled to KLH
