Chloride is a critical component of all living cells.
Voltage-gated chloride channels regulate cellular traffic of chloride ion.
The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport.
Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis.
In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb).
Additional forms of these proteins are obtained by alternative splicing.
All CLC proteins (~700-1000aa) are predicted to contain 10 (possibly 12) transmembrane domains.
Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues.
Rat CLC-3 is 760aa membrane protein (human CLC-3 760 or 820aa; mouse CLC-3 760/818aa) (1).
Alternatively spliced short forms of mouse and human CLC-3 have also been found.
CLC-3 is abundant in brain (olfactory, hippocampus, and cerebellum) and moderate levels are found in the lung, kidney, and adrenals.
CLC-3 has been implicated in neuronal cell function through regulation of membrane excitability by protein kinase C.
It could also help neuronal cells to establish short-term memory.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:C5837-10G: CLC3, Rat, Control Peptide (Clcn3, Chloride Channel)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes rat CLC3. No significant sequence homology is detected with other CLCs.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 18aa from the C-terminal, extracellular domain of rat CLC3. Species Sequence Homology (100%): mouse, guinea pig, rat, human, rabbit, frog.
