Cocaine- and Amphetamine Related Transcript (CART) was initially identified using PCR differential display as mRNA whose levels in the brain was specifically induced by psychomotor stimulants such as cocaine and amphetamine (1).
CART encodes a secretory single chain polypeptide of 129aa or 116aa protein due to the usage of alternate splicing and processing.
The first 27aa represents the hydrophobic core indicative of signal peptide.
The CART protein also has several basic amino acids near the 54-66 residues with a potential to form multiple proteolytic forms.
CART mRNA was specially enriched in the hypothalamus.
In the striatum, CART expression was induced 4-5 fold by cocaine and amphetamine.
CART is highly conserved between human and mouse (95% amino acid homology); CART gene was localized to human chromosome 5 (2).
Most recently, CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY.
Starvation decreases CART levels in the arcuate nucleus (3).
Obese animals have virtually no CART.
Peripheral administration of leptin in obese animals induces CART.
Intracereberoventricular injection of recombinant CART inhibits both normal and starvation-induce feeding, and completely inhibits NPY-induce feeding.
Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake.
CART 55-102 (numbers corresponds to the predicted signal-peptide-cleavage site in the long form of CART) was physiologically active in inhibiting feeding in normal rats (3).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL
ELISA: 1:10,000-50,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：chicken
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes mouse CART. 100% conserved in rat and sheep and 97% in human CART. Antibody crossreactivity in various species is not known
Isotype：IgY
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to aa55-102 from Mouse/Rat CART. (KLH)
