In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step.
The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation.
Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK).
Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism.
Mitochondrial oxidation of LC-FCA is initiated by the sequential action of CPT-1, which is located in the outer membrane, and CPT-2, which is located in the inner membrane together with a carnitine-acylcarnitine translocase.
CPT-2 (mouse/rat/human 658-aa, ~74kD, chromosome 1p32, ~20% identity with CPT1) is a ubiquitous malonyl-CoA-insensitive transferase localized in the inner mitochondrial membrane.
It catalyzes the re-synthesis of acyl-CoA from acyl-carnitines.
CPT-2 deficiency leads to the most commonly inherited, lipid myopathy in adults characterized by exercise-induced pain, stiffness, and myoglobinuria.
The aa sequences of the two isoforms are ~61% identical.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Specificity：Recongizes mouse CPT-2
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：16-aa peptide sequence mapping near the C-terminus of mouse CPT-2L (KLH coupled). Species Sequence Homology: rat (93%); human (86%). No significant sequence homology is seen with CPT-1 or any other protein.
