In cells and organisms, the excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA.
In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1).
Activities of ACC-1 and ACC-2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK).
Diabetes deranges AMPK master-switch, represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism.
Cytosolic LCFA-CoA is converted into acyl-carnitine at mitochondrial membrane surface by carnitine palmitoyl transferase-1 (CPT-1) followed by the reconversion of the latter into LCFA-CoA by CPT-2 in the mitochondrial matrix.
Mitochondrial oxidation of LC-FCA is initiated by the sequential action of CPT-1, which is located in the outer membrane, and CPT-2, which is located in the inner membrane together with a carnitine-acylcarnitine translocase.
CPT-1 liver or CPT1A or LCPT-1 (mouse 764aa, rat 773aa, human 773aa, ~88kD, chromosome 11q13) is malonyl-CoA-sensitive enzyme localized on the outer surface of mitochondrial 'contact sites'.
It catalyzes the conversion of long-chain acyl-CoA into acyl-carnitine, committing the acyl moiety to intramitochondrial oxidation.
It is predominantly expressed in kidney, liver and in trace amounts in heart.
The 'muscle' isoform CPT1B or CPT1M or MCPT-1 (mouse/rat/human 772aa, chromosome 22q13.3) is found in heart, skeletal muscle, adipose tissue and brain.
The amino acid sequences of the two isoforms are ~61% identical.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000.
Coat plates with 50-100ng of C1385-48: Carnitine Palmitoyl Transferase 1, Muscle, Mouse, Control Peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes mouse Carnitine Palmitoyl Transferase 1, Muscle (CPT1-M). No significant sequence homology is seen with LCPT-1, CPT-2 or any other protein.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 13aa near the C-terminus of mouse CPT1-M conjugated to KLH. Swiss/UniProt Accession: Q63704. Species Sequence Homology: rat; 92%, human; 69%.
