In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA.
In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step.
The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation.
Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMPactivated protein kinase (AMPK).
Diabetes deranges AMPK master-switch and represses the ACC-1 gene expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism.
Mitochondrial oxidation of LC-FCA is initiated by the sequential action of CPT-1, which is located in the outer membrane, and CPT-2, which is located in the inner membrane together with a carnitine-acylcarnitine translocase.
CPT-1 liver or CPT1A or LCPT-1 (mouse 764-aa, rat 773-aa, human 773-aa, ~88-kda, chromosome 11q13) is malonyl-CoA-sensitive enzyme localized on the outer surface of mitochondrial 'contact sites'.
It catalyzes the conversion of long-chain acyl-CoA into acyl-carnitine, committing the acyl moiety to intramitochondrial oxidation.
It is predominantly expressed in kidney, liver and in trace amounts in heart.
The 'muscle' isoform CPT1B or CPT1M or MCPT-1 (mouse/rat/human 772-aa, chromosome 22q13.3) is found in heart, skeletal muscle, adipose tissue and brain.
The aa sequences of the two isoforms are ~61% identical.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes mouse Carnitine Palmitoyl Transferase 1, Muscle (CPT1-M). No significant sequence homology is seen with LCPT-1, CPT-2 or any other protein. Species Crossreactivity: rat, 92%; human, 69%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide consisting of 13aa sequence mapping near the C-terminus of mouse CPT1-M conjugated to KLH.
