In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA.
In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step.
The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation.
Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK).
Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism.
Mitochondrial oxidation of LC-FCA is initiated by the sequential action of CPT-1, which is located in the outer membrane, and CPT-2, which is located in the inner membrane together with a carnitine-acylcarnitine translocase.
CPT-1 liver or CPT1A or LCPT-1 (mouse 764-aa, rat 773-aa, human 773-aa, ~88-kda, chromosome 11q13) is malonyl-CoA-sensitive enzyme localized on the outer surface of mitochondrial 'contact sites'.
It catalyzes the conversion of long-chain acyl-CoA into acyl-carnitine, committing the acyl moiety to intramitochondrial oxidation.
It is predominantly expressed in kidney, liver and in trace amounts in heart.
The 'muscle' isoform CPT1B or CPT1M or MCPT-1 (mouse/rat/human 772-aa, chromosome 22q13.3) is found in heart, skeletal muscle, adipose tissue and brain.
The aa sequences of the two isoforms are ~61% identical
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:10,000-1:100,000.
Using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher
Control Peptide:C1385-31: Carnitine Palmitoyl Transferase 1, Liver, Mouse,
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes mouse CPT1-L.. No significant sequence homology is seen with MCPT-1, CPT-2 or any other protein.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 13aa of mouse CPT1-L at C-terminal. Species sequence homology: rat (84%) and human (69%)
