Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase (PIK3).
Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated on Thr308 in the catalytic domain and Ser473 in the C-terminal regulatory domain.
Several protein partners for PKB have been identified.
CTMP, a carboxyl terminal modulator protein, binds to PKB-a and negatively regulates PKB activity by inhibiting phosphorylation of these PKB residues.
CTMP (mouse 248aa, human 240aa, chromosome 1q21, 22-26kD) predominantly expressed in skeletal muscle, testis, uterus, brain, and kidney, with lower levels in heart, liver, and lung.
The presence of multiple transcripts suggested that CTMP undergoes alternative splicing.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human CTMP. Species Crossreactivity: The human peptide sequence is 100% conserved in mouse CTMP.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo
Immunogen：A 17aa peptide sequence near the C-terminus of human CTMP
