Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase (PIK3).
Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated on thr308 in the catalytic domain and ser473 in the C-terminal regulatory domain.
Several protein partners for PKB have been identified.
CTMP, a carboxyl terminal modulator protein, binds to PKB-a and negatively regulates PKB activity by inhibiting phosphorylation of these PKB residues.
CTMP (mouse 248-aa, human 240-aa, chromosome 1q21, 22-26kD) predominantly expressed in skeletal muscle, testis, uterus, brain, and kidney, with lower levels in heart, liver, and lung.
The presence of multiple transcripts suggested that CTMP undergoes alternative splicing.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The human control peptide sequence is 100% conserved in mouse CTMP. Antibody crossreactivity in various species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 17-aa peptide sequence near the middle region of human CTMP (1) was synthesized, coupled to KLH
