Cannabinoids, a group of C21 compounds present in Cannabis sativa L., their carboxylic acids, analogs, and transformation products, are the active ingredients found in hasish and marihuana.
(-)-trans-D9-tetrahydrocannabinol (D9-THC) is the major psychopharmacologically active component of cannabis.
Cannabis affects cognition and memory, euphoria and sedation, and antinociception (analgesia) without the respiratory depression problems associated with opioid analgesics.
To date, two sub-types of the G-protein coupled cannabinoid receptor, CB1 and CB2, have been identified.
CB1 (rat/mouse 473 aa, human 472 aa, ~95% sequence homology) has 7 TM domains with an extracellular N-terminus and cytoplasmic C-terminus.
An alternative spliced N-terminal variant, CB1A, has also been reported.
The CB1 receptor has been shown to inhibit adenylate cyclase activity in a pertussis-toxin sensitive manner and to be more responsive to psychoactive cannabinoids than to non-psychoactive cannabinoids.
CB1 also couples to a pertussis-toxin sensitive G-protein to regulate Ca2+ currents, to activate inward rectifying K+ channels, and to activate MAP Kinases.
CB1 receptor is distributed throughout the brain, with higher representation in the hippocampus, a brain region essential for storage of newly acquired information and changes of mood and behavioral state.
In the cerebellum, CB1 is most dense in the molecular layer, consistent with the effects of cannabinoids on the coordination of motor function and motor learning.
Outside the CNS, CB1 expression is found in testes, endothelial cells, and ileum longitudinal smooth muscle.
Applications:Suitable for use in Western Blot and ELISA.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 (ECL)ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: C1075-70A
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid with 0.05% sodium azide and 40% glycerol.
Specificity：Recognizes human Cannabinoid Receptor 1 (CB1). Species crossreactivity: Human.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 14 amino acid peptide sequence near the extracellular, N-terminus of human CB1 (KLH conjugated). Species Sequence Homology: Rat, mouse, feline, monkey, zebra finch, salamander (100%), pufferfish (85%). The immunogen shows no significant sequence homology with CB2 or other receptors.
