Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types.
Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle.
A novel family of striated muscle-specific calcineurin-interacting proteins called calsarcins or myozenins has been identified that interact and colocalize with the Z-disc protein alpha-actinin.
Two isoform of calsarcins, Calsarcin-1 and Calsarcin-2, with specific expression pattern have been identified in human, rat and mouse.
Calsarcins tether calcineurin to the sarcomere of cardiac and skeletal muscle.
Besides calcineurin and ?-actinin, calsarcins interact with other Z-disc proteins ?-filamin, telethonin and TCAP.
Because calcineurin responds to sustained, low amplitude calcium signals, calsarcins may serve to localize calcineurin in the vicinity of unique intracellular pool, where it can interact with specific upstream activators or downstream substrates.
Therefore, calsarcins may play an important role in modulating the function and substrate specificity of calcineurin in striated muscle cells.
Calsarcin-1 (CALS-1), also known as Myozenin-2 or MYOZ2, is a ~32kD protein (human/mouse 264-aa, ~88% identity).
Amino acids 217-240 of calsarcin-1 are necessary for its interaction with a-actinin-2 in the Z-disc of sarcomeric muscle fibers.
It is expressed, throughout the development-cycle, in all striated muscle tissues.
However, CALS-1 expression is localized in slow-twitch fibers (soleus and plantaris), which depends on chronic motor neuron stimulation that results in sustained cellular calcium.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The peptide is 82% conserved in human calsarcins-1 or Myozenin-2. No significant sequence homology is seen with calsarcins-2 or other proteins. Antibody reactivity in various species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：A 17-aa peptide sequence from the C-terminus of mouse Calsarcins-1 (1) was synthesized, coupled to KLH
