In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA.
In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step.
The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation.
Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMPactivated protein kinase (AMPK).
Diabetes deranges AMPK master-switch and represses the ACC-1 gene expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide well
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:C1385-31: Carnitine Palmitoyl Transferase 1, Liver, Mouse, Control Peptide (CPT1-L)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes mouse Carnitine Palmitoyl Transferase 1, Liver (CPT1-L). No significant sequence homology is seen with MCPT-1, CPT-2 or any other protein.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：A 13-aa peptide sequence mapping near the C-terminus of mouse CPT1-L (KLH). Species sequence homology: rat 84% and human 69%.
