Calcium (Ca+2 or Ca) is the most abundant cation and it is required for many physiological activities such as bone formation and it acts as a second messenger in signal transduction.
However only 1% of Ca is present in ionic form in biological fluids.
Ca concentration is regulated by calcitropic hormones that act on bone, kidney, and intestine.
Extracellular Ca+-levels are sensed and regulated by Calcium Sensing receptor (CASR).
When Ca levels are limiting then it must be taken up by active, transcellular pathways comprising (1) Ca++ entry across apical membrane, (2) cytosolic transport of Ca++ across the cell form apical to basolateral membrane facilitated by a family of low mol wt Calcium binding proteins (CABPs) that include vitamin D3-dependent Ca++ binding proteins (calbindin-D9k, Calbindin-28k, Calretinin, Parvalbumin, S100, calmodulin) and finally (3) an active extrusion of Ca++ through basolateral membrane mediated by Ca++-ATPase and Na+-Ca++ exchangers (NCX).
Ca++ absorption in intestine and its reabsorption in kidney are carried out by Ca++ Transport (CaT) proteins, CaT-1, CaT-2 or Epithelial Ca Channel (ECAC1/ECAC2/CaT-Like (CaT-L) proteins.
Applications:Suitable for use in ELISA, Western Blot, Antibody Blocking, Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000.
The expected size of Cat-1 is ~83kD.
However, posttranslational modification may alter its size depending upon the species and cell type
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Antibody Blocking: use 5-10ug control peptide per 1ul antiserum to confirm antibody specificity.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Neat serum
Form：Supplied as a liquid in 40% glycerol.
Specificity：The peptide is 100% conserved in rat, mouse and 94% in Cat-1/htrp8A and htrp8b proteins. No significant sequence homology is seen with CaT-2 or other proteins. Antibody reactivity in various species is not known.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：A 18-aa peptide sequence, mapping within cytoplasmic N-terminus of rat CaT-1/ECAC2, coupled to KLH.
