The divalent cations (Ca2+ and Mg2+) are important for a multitude of cellular functions.
A G-protein coupled receptor, termed calcium sensing receptor, responds to extracellular calcium and mediates several of the known effects of Ca on parathyroid and renal functions.
CaSR is expressed in a number of tissues including thyroid C-cells.
Brain, and GI tract.
Ca2+ acts as first messenger via CaSR-mediated activation of second messenger systems (e.g., phospholipase C and A2, cAMP) altering cellular physiology.
Several mutations in the human CaSR gene have been identified in several inherited diseases of calcium metabolism such as familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism(NSHPT), and autosomal dominant hypocalcemia (ADH).
Human CaSR is a 1078aa (Rat/mouse 1079aa) 7 transmembrane protein characterized by the presence of a single peptide, a large extracellular (~592aa) N-terminus and cytoplasmic C-terminus.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat plates at 1ug/ml
Immunofluorescence: 1-20ug/mlImmunohistochemistry (Paraformaldehyde fixed): 1-20ug/mlOptimal dilutions to be determined by the researcher.
Control peptide:C0117-10: Calcium Sensing Receptor, Human, Control Peptide (CASR)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes human CaSR. No significant sequence homology with other GPCR.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 20aa sequence near the C-terminus, cytoplasmic domain of human CaSR, conjugated to KLH. Species Sequence Homology: mouse and rat: 90%; bovine: 85%.
