84-1041-33　［取扱停止］Calcium Sensing Receptor (CASR) 100ul　C0117-05

特徴

• The divalent cations (Ca2+ and Mg2+) are important for a multitude of cellular functions.
• A G-protein coupled receptor, termed Calcium sensing receptor, responds to extracellular calcium and mediates several of the known effects of Ca on parathyroid and renal functions.
• CaSR is expressed in a number of tissues including thyroid C-cells.
• Brain, and GI tract.
• Ca2+ acts as first messenger via CaSR-mediated activation of second messenger systems (e.g., phospholipase C and A2, cAMP) altering cellular physiology.
• Several mutations in the human CaSR gene have been identified in several inherited diseases of calcium metabolism such as familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalcemia (ADH).
• Human CaSR is a 1078aa (Rat/mouse 1079aa) 7 transmembrane protein characterized by the presence of a single peptide, a large extracellular (~592aa) N-terminus and cytoplasmic C-terminus.
• CASR has been documented in bone marrow, brain, breast (mostly cancer), colon, kidney, parathyroid, skin, stomach, and thyroid.
• ESTs have been isolated from normal kidney and gallbladder, in addition to parathyroid cancer libraries.
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 (ECL)
• ELISA: 1:10,000-1:50,000.
• Control peptide (C0117-10) can be used to coat ELISA plates at 1ug/ml.
• See references 8, 9.
• Optimal dilutions to be determined by the researcher.
• Control peptide:C0117-10: Calcium Sensing Receptor, Human, Control Peptide (CASR)
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.