84-1041-28　［取扱停止］Calcineurin B (CnB; Protein Phosphatase 2B, B subunit) 100ul　C0114-51

特徴

• Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types.
• Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle.
• A novel family of striated muscle-specific calcineurin-interacting proteins called calsarcins or myozenins has been identified that interact and colocalize with the Z-disc protein alpha-actinin.
• Two isoform of calsarcins, Calsarcin-1 and Calsarcin-2, with specific expression pattern have been identified in human, rat and mouse.
• Calsarcins tether calcineurin to the sarcomere of cardiac and skeletal muscle.
• Besides calcineurin and a-actinin, calsarcins interact with other Z-disc proteins filamin, telethonin and TCAP.
• Because calcineurin responds to sustained, low amplitude calcium signals, calsarcins may serve to localize calcineurin in the vicinity of unique intracellular pool, where it can interact with specific upstream activators or downstream substrates.
• Therefore, calsarcins may play an important role in modulating the function and substrate specificity of calcineurin in striated muscle cells.
• Calcineurin (also known as CALNA or CALNA1, Calcineurin-alpha, Protein phosphatase 2B or PP2B) is the Ca+/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in from yeast to mammals.
• It is a heterodimers of two subunits: Calcineurin B/CnB, the 19-kda Ca+-binding and regulatory subunit, and Calcineurin A/CnA, ~61-kda catalytic subunit that is highly homologous with PP1 and PP2A.
• Multiple catalytic subunits of calcineurin are derived from at least 2 structural genes, type 1 (calcineurin A-alpha) and type 2 (calcineurin A-beta, CALNA2), each of which can produce additional alternatively spliced transcripts.
• CnB belongs to the family of EF-hand Ca-binding proteins.
• Both CnB and calmodulin are important for the activation of the phosphatase activity of calcineurin.
• Calcineurin controls the production of many cytokines including IL-2, TNF-alpha in the T-cell activation pathway.
• Calcineurin mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is required for NF-AT activation, nuclear translocation, and subsequent gene expression in T-cells.
• The immunosuppressive drugs, such as FK506, inhibit activation of NF-AT by calcineurin.
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000.
• B subunit is ~19kD
• ELISA: 1:10,000-1:100,000Optimal dilutions to be determined by the researcher.
• Western Blot Control Peptide: C0114-52
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.