Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types.
Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle.
A novel family of striated muscle-specific calcineurin-interacting proteins called calsarcins or myozenins has been identified that interact and colocalize with the Z-disc protein alpha-actinin.
Two isoform of calsarcins, Calsarcin-1 and Calsarcin-2, with specific expression pattern have been identified in human, rat and mouse.
Calsarcins tether calcineurin to the sarcomere of cardiac and skeletal muscle.
Besides calcineurin and ?-actinin, calsarcins interact with other Z-disc proteins ?-filamin, telethonin and TCAP.
Because calcineurin responds to sustained, low amplitude calcium signals, calsarcins may serve to localize calcineurin in the vicinity of unique intracellular pool, where it can interact with specific upstream activators or downstream substrates.
Therefore, calsarcins may play an important role in modulating the function and substrate specificity of calcineurin in striated muscle cells.
Calcineurin (also known as CALNA or CALNA1, Calcineurin-alpha, Protein phosphatase 2B or PP2B) is the Ca+/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in from yeast to mammals.
It is a heterodimer of two subunits: Calcineurin B/CnB, the 19-kda Ca+-binding and regulatory subunit, and Calcineurin A/CnA, ~61-kda catalytic subunit that is highly homologous with PP1 and PP2A.
Multiple catalytic subunits of calcineurin are derived from at least 2 structural genes, type 1 (calcineurin A-alpha) and type 2 (calcineurin A-beta, CALNA2), each of which can produce additional alternatively spliced transcripts.
CnB belongs to the family of EF-hand Ca-binding proteins.
Both CnB and calmodulin are important for the activation of the phosphatase activity of calcineurin.
Calcineurin controls the production of many cytokines including IL-2, TNF-alpha in the T-cell activation pathway.
Calcineurin mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is required for NF-AT activation, nuclear translocation, and subsequent gene expression in T-cells.
The immunosuppressive drugs, such as FK506, inhibit activation of NF-AT by calcineurin.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
A subunit is ~61kD and B subunit is ~19kD
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Westen Blot Positive Control: C0114-25
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes A and B subunits of bovine Calcineurin. The antibody is expected to react with mouse, rat and human calcineurin A and B subunits. The use of purified bovine brain calcineurin is recommended for the identification of appropriate bands in Western.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo
Immunogen：Calcineurin purified from bovine brain (contains both A and B subunits)
