84-1041-26　［取扱停止］Calcineurin A (CnA, Protein Phosphatase 2B, A subunit) 100ug　C0114-06

特徴

• Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types.
• Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle.
• Calcineurin (also known as CALNA or CALNA1, Calcineurin-alpha, Protein phosphatase 2B or PP2B) is the Ca+/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in from yeast to mammals.
• It is a heterodimers of two subunits: Calcineurin B/CnB, the 19-kda Ca+-binding and regulatory subunit, and Calcineurin A/CnA, ~61-kda catalytic subunit that is highly homologous with PP1 and PP2A.
• Multiple catalytic subunits of calcineurin are derived from at least 2 structural genes, type 1 (calcineurin A-alpha) and type 2 (calcineurin A-beta, CALNA2), each of which can produce additional alternatively spliced transcripts.
• CnB belongs to the family of EF-hand Ca-binding proteins.
• Both CnB and calmodulin are important for the activation of the phosphatase activity of calcineurin.
• Calcineurin controls the production of many cytokines including IL-2, TNF-alpha in the T-cell activation pathway.
• Calcineurin mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is required for NF-AT activation, nuclear translocation, and subsequent gene expression in T-cells.
• The immunosuppressive drugs, such as FK506, inhibit activation of NF-AT by calcineurin.
• Human calcineurin A-alpha (~60kD) and calcineurin B (~19kD) were co-expressed with yeast myristoyl-CoA:protein N-myrisoyltransferase and purified to >95%.
• Recombinant and purified Calcineurin is N-myristoylated on the CnB, as in the natural protein, resulting into biologically active protein.
• For western blot +ve control , calcineurin is supplied in SDS-PAGE sample buffer (reduced).
• Load ~10 ul/lane to visualize with antibodies.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
• B subunit is ~19kD
• ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.