Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
D28K is ~28kD.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Control peptide, because of its low MW (<3kD), is not suitable for Western.
It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1ug of affinity purified IgG or 1ul antiserum) to confirm antibody specificity.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Affinity purified over the control-peptide Sepharose
Form：Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Specificity：The peptide is 95% conserved in rat, human, rabbit, chicken, bovine D28K. No significant sequence homology is seen with D9K, calretinin or other CABPs.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：A 20-aa peptide sequence, within the C-terminal, non-calcium binding domain of mouse D28K (1) was synthesized, coupled to KLH
