Calcium is the most abundant cation and is required for many physiological activities and acts as a second messenger in signal transduction.
Only 1% of Ca is present in ionic form in biological fluids.
Ca concentration is regulated by calcitropic hormones that act on bone, kidney and intestine.
Extracellular Ca+- levels are sensed and regulated by Calcium Sensing receptor (CASR).
When Ca levels are limiting then it must be taken up by active, transcellular pathways.
Calbindins are Ca-binding proteins belonging to the troponin C superfamily.
There are two types of CaBPs: the “trigger” and the “buffer” CaBPs.
The conformation of “trigger” type CaBPs changes upon Ca2+ binding and exposes regions on proteins that interact with target molecules, thus altering their activity.
The buffer-type CABP are thought to control the intracellular calcium concentration.
CABP9K (mouse, rat and human 79aa; chromosome Xp; ~9kD) is a cytosolic Ca-binding protein initially found in rat pancreas.
It is also expressed in intestine, placenta, uterus and kidney.
Its expression is controlled by vitamin D and sex hormone in a tissue specific manner.
In keeping with its role in Ca- transport, its expression is highest in duodenal villus enterocytes.
It is further shown that CABP9K is only expressed in differentiated enterocytes.
CABP9K gene also contains Cdx2-homeoprotein binding sites, and that Cdx2 may play a crucial role in CABP9K transcription.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000 using 50-100ng C0104-60 Control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.09% sodium azide
Specificity：Recognizes mouse CABP9K at ~9kD. No significant sequence homology is seen with D28K or other CABPs.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 16aa within the N-terminal Ca-binding domain of mouse CABP9K. Species Sequence Homology: rat; 82%, porcine; 75%, bovine; 62%, human; 56%
