Bombesin (BN) is a tetradecapepide (Pyro-ENR LGN QWA VGH LM-amide) that was originally isolated from frog skin.
Several BN-related peptides have been isolated from amphibians and classified into three subfamilies: Bombesin, ranatensin, and phyllolitorin based upon the last three C-terminal residues.
Two BN-like peptides have been identified in mammals: NMB (neuromedin B) in the ranatensin family and GRP (gastrin-releasing peptide) in the BN-family.
BN-family of G-protein coupled receptors include at least four receptor subtypes: The GRP-preferring receptors (GRP-R or bb2), the NMB-preferring receptors (NMB-R or bb1) and Bombesin receptor subtype 3 (BRS-3 or bb4).
These receptors share approx.
50% amino acid homology and bind bombesin.
However, BRS-3 has much lower affinity for BN than GRP-R and NMB-R.
Mammalian BN-like peptides are widely distributed in the brain and gastrointestinal tract, where they modulate smooth-muscle contraction, exocrine and endocrine activities, metabolism and behavior.
Most recently, BRS-3 deficient mice have been shown to develop mild obesity, associated with hypertension, and impairment of glucose metabolism, reduced metabolic rate, increased feeding efficiency and subsequent hyperphagia.
Human BRS-3 gene (chromosome x) encodes a 399 aa protein with seven transmembrane domains.
The NH2 and COOH-termini are predicted to be extracellular and cytoplasmic, respectively.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes human Bombesin Receptor 3 (BRS-3).
Isotype：IgG
Calc Crossreactivity：Hu
Immunogen：BRS-3 specific peptide sequences from the C-terminal cytoplasmic domain.
