84-1040-97　BACE, Asp2 (Memapsin2, Beta-site APP Cleaving Enzyme, Aspartyl Protease 2) 100ul　B0002-88

特徴

• Beta-amyloid (Ab) deposition in the brain is the hallmark of Alzheimer's Disease (AD).
• To initiate Ab formation, b-secretase cleaves APP at the N-terminus of Ab to release APPsb (~100 kDsoluble NT-fragment), and C99, a 12-kD CT membrane fragment.
• Alternatively, a-secretase cleaves within the Ab to prevent the formation of Ab.
• Cleavage by a-secretase produces a soluble Nterminal fragment, APPsa, and a 10-kD membrane C-terminal fragment, C83.
• Both C99 and C83 can be further cleaved by gsecretase releasing Ab and a nonpathogenic p3 peptide, respectively.
• Recently, BACE (Beta-site APP Cleaving Enzyme) has been identified as b-secretase.
• BACE belongs to the family of Aspartyl proteases (Asp) also known as Memapsins.
• At least four related Asps, located on chromosome IV and X, have been cloned (Asp1, Asp2, Asp3, and Asp4).
• Human BACE/Asp2/Memapsin2, located on chromosome 11, is a trans- membrane protein of 501 aa (signal peptide 1-21 aa, a proprotein domain 22-45 aa, 1 TM domain near the CT, and a short cytoplasmic CT- tail of 24 aa; mature protein 46-460 aa)
• BACE expression was most prominent in most areas of the rat brain and pancreas.
• It has been localized in the compartments of the secretory pathways.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000ELISA:1:10,000-50,000.
• Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.