84-1040-95　Aven (Apoptosis Caspase Activation Inhibitor, Cell Death Regulator Aven, PDCD12) 100ul　A4445-11G

特徴

• Apoptosis or programmed cell death is a fundamental cellular process that is essential for normal tissue development and abnormal growth such as cancer, neurodegeneration, autoimmune diseases, and angiogenesis, etc.
• Apoptosis is driven by two classes of specialized proteases known as caspases (Cysteine Aspartase).
• The initiator caspases can be activated by self-cleavage.
• The effector caspases are then activated in an amplification cascade.
• Mitochondria play a pivotal role in the regulation of apoptosis.
• Several key factors are released from the mitochondria that regulate apoptosis.
• The first such factor (Cytochrome-C) to be described binds to a cytoplasmic scaffolding protein called Apaf-1.
• Binding of the mitochondria factor allows Apaf-1 to form a ternary complex with, and activate, the initiator pro-caspase-9.
• Active caspase-9 then turns on downstream effector caspases, initiating apoptosis.
• Several key proteins regulate apoptosis by suppressing (e.g., Bcl-2 and related family of proteins) or promoting (e.g., AIF etc) apoptosis pathway.
• Bcl-XL is a known anti-apoptotic protein that acts at a late post-mitochondrial step.
• A new intracellular membrane protein, Aven (from Aventine, a Roman stronghold) has now been shown to bind both Bcl-XL and Apaf-1.
• Aven (mouse 342aa, human 362 aa) is a conserved protein that has broad tissue distribution with prominent expression in heart, skeletal muscle, kidney, liver, pancreas, testis, and several established cell lines (HeLa, IB4, and Raji).
• Aven interferes with the ability of Apaf-1 to self-associate, and subsequent inhibition of Apaf-1 mediated activation of caspases.
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using ECL
• ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure.
• Control peptide can be used to coat ELISA plates at 1ug/ml.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.d sections of tissues may be tested.