Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain.
Proton-gated cation channels are activated by low pH in nociceptive neurons.
H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC), (3) and mechanosensory channel proteins of nematode degenerins (DEG).
NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop.
All members of this family are selective for Na+ and blocked by amiloride
The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASIC for Acid Sensing Ion Channels).
Three are at least three distinct proteins in ASIC family: ASIC1 (identical with human BNAC2 or BNC2), expressed in brain and dorsal root ganglions (DRG) cells, is activated by pH <7.0.
A splice variant of rat ASIC, ASIC-beta, is expressed only in a subset of small and large diameter sensory neurons and absent in sympathetic neurons and CNS.
MDEG1/ASIC2, 67% identity with ASIC1, requires more acidic pH than ASIC1 and has slower activation kinetic.
MDEG2/ASIC2b), a splice variant of MDEG1, is expressed in both brain and sensory neurons.
MDEG2 is activated neither by mutations nor low pH.
However, it acts as modulatory subunit when associated with MDEG1 and another H+-activated channel, DRASIC/ASIC3 (Dorsal Root ganglion ASIC).
DRASIC is specific for sensory neurons.
In response to a drop in pH, DRASIC gives rise to a biphasic currents with poor discrimination between Na+ and K+.
This sustained current may be important in pain sensation.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
ELISA: 0.5-1ug/ml coat ELISA plates at 1ug/ml with Control Peptide
Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in 100mM Tris, pH 7.5 and 0.2% BSA, 0.02% sodium azide, 40% glycerol.
Specificity：Rat control peptide is 95% conserved in human MDEG1/BNaC1/BNA1. No significant sequence homology is detected with other ASICs. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Rt
Immunogen：20aa Peptide sequence near the extracellular, N-terminus, of rat ASIC-b
