Water is a critical component of all living cells.
Interestingly, tissue membranes show a great degree of water permeability.
Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin".
Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues.
A new member of AQP family, AQP10, has been cloned (1).
AQP10 (human 264 aa) is most closely related with AQP3 (53%), AQP9 (52%), and AQP7 (43%).
It is abundantly expressed in duodenum and jejunum.
AQP10 is not permeable to urea and glycerol.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Note: We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands
Western Blot: 1-10ug/ml using ECL
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:A3000-70 Aquaporin 10, Human, Control Peptide (AQP10)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes human AQP10. The 17aa peptide is unique to AQP10 without significant homology to any other AQPs. AQP10 from other species are yet to be cloned.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A 17aa synthetic peptide within the cytoplasmic, C-terminal region of human AQP10 (1) coupled to KLH
