84-1040-83　［取扱停止］Aquaporin 10 (AQP10) 100ul　A3000-75

特徴

• Water is a critical component of all living cells.
• Interestingly, tissue membranes show a great degree of water permeability.
• Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
• Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin".
• Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues.
• A new member of AQP family, AQP10, has been cloned (1).
• AQP10 (human 264aa) is most closely related with AQP3 (53%), AQP9 (52%), and AQP7 (43%).
• It is abundantly expressed in duodenum and jejunum.
• AQP10 is not permeable to urea and glycerol.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:50,000; Control peptide (A3000-70) can be used to coat ELISA plates at 1ug/ml
• Western Blot: 1:1000-1:5000
• Note: We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
• Optimal dilutions to be determined by the researcher.
• Control Peptide: A3000-70 Aquaporin 10, Human, Control Peptide (AQP10)
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.