Water is a critical component of all living cells.
Interestingly, tissue membranes show a great degree of water permeability.
Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin".
Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues.
AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin.
The lens specific AQP0 represents up to 80% of total lens membrane protein.
Defects in MIP26 are cause of autosomal dominant cataract.
The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP.
The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence technique
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat ELISA plates at 1ug/ml
Note: We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a powder lyophilized from a PBS solution containing 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes human AQP6.
Isotype：IgY
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated 19-amino acid synthetic peptide within the C-terminal domain of human AQP6. Epitope Location: C-terminal, cytoplasmic domain. Note: The epitope is not found in isoform 2. Species Sequence Homology: rat: 47%
