Water is a critical component of all living cells.
Tissue membranes show a great degree of water permeability.
Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by "aquaporin".
Genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues.
AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporins.
The lens specific AQP0 represents up to 80% of total lens membrane protein.
Defects in MIP26 are cause of autosomal dominant cataract.
The cataract Fraser mutation (CAT-FR or Shriveled) is a trans- poson-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP.
The lens opacity mutation (LOP) is an aa substitution that inhibits targeting of MIP to the cell membrane.
AQP5 (282aa) is found in several epithelial tissues (salivary and lachrymal glands, corneal epithelium in the eye and lung).
It is implicated in generation of saliva, tears, and pulmonary secretions.
AQP families of proteins are predicted to contain six transmembrane domains.
Rat-AQP5 is a 265 amino acid transmembrane protein.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
ELISA: 1:10,000-1:50,000 Coat with 1ug/ml Control Peptide.
Optimal dilutions to be determined by the researcher.
Control peptide:A3000-23: Aquaporin 5, Rat, Control Peptide (AQP5)
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity：Reconizes rat Aquaporin 5 (AQP5). No significant homology is seen with other AQPs or any other protein.
Isotype：IgG1
Calc Applications Abbrev：E
Calc Crossreactivity：Mo Rt
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of 17 amino acids in the C-terminal region of rat Aquaporin 5. Species Sequence Homology: mouse (100%); human (80%); sheep (76%).
