84-1040-71　Aquaporin 3 (AQP3, GLIP) 100ul　A3000-11D

特徴

• Water is a critical component of all living cells.
• Interestingly, tissue membranes show a great degree of water permeability.
• Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
• Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin".
• Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues.
• AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin.
• The lens specific AQP0 represents up to 80% of total lens membrane protein.
• Defects in MIP26 are cause of autosomal dominant cataract.
• The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the C-terminus of MIP.
• The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane.
• AQP3 (GLIP, Glycerol-transporting integral protein; 285aa; 31.4kD) has a wide tissue distribution.
• AQP3 may function as a water and urea exit mechanism in antidiuresis in collecting duct cells.
• It is also slightly permeable to urea and glycerol.
• AQP3 may play an important role in gastrointestinal tract water transport and in glycerol metabolism.
• In the kidney it is predominantly expressed in the inner medulla.
• AQP families of proteins are predicted to contain six transmembrane domains.
• The N and C-termini are predicted to be cytoplasmic.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100,000.
• using 50-100ng control peptide (Cat #A3000-11G)/well
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.