84-1040-65　APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1) 100ul　A2298-74D

特徴

• Reactive oxygen species (H2O2·, OH·, O2, HOCL) generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents can induce a wide variety of lesions in DNA ranging from oxidized bases (8-oxoguanine and thymine glycol), base loss and DNA strand breaks with modified ends such as 3'-phosphate and 3'-phopshoglycolate.
• Unrepaired damaged/modified DNA has been implicated in aging and cancer.
• Many proteins that are involved in BER (base excision repair) have been identified in E.
• coli and mammals.
• Repair of modified is initiated by their removal by specific DNA glycosylase resulting in AP (apurinic/apyrimidinic) sites.
• A series of steps starting with the cleavage of the DNA strand adjacent to AP sites, removal of the deoxyribose phosphate residue, followed by gap filling synthesis and ligation.
• The DNA strand cleavage is catalyzed by a variety of AP-endonucleases (APE or HAP1, APEX, APE-1) that cleave 5' to the AP sites.
• APE also has an associated 3'-endonuclease activity the removes 3'trans-a,b-unsaturated aldehyde generated by AP lyase, as well as 3'-phosphate and 3'-phosphoglycolate.
• ROS generated 3'-blocked termini at the site of strand breaks are also removed.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000ELISA:1:10,000-50,000.
• Control peptide can be used to coat ELISA plates at 1ug/ml.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.