84-1040-62　［取扱停止］Anion Exchanger 4 (AE4) 100ul　A2295-14G

特徴

• Anion exchangers (AE) are membrane proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport.
• AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification.
• AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3).
• Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues.
• AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin.
• The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1.
• In fact, AE1 N-terminus is 300aa shorter than both the AE2 and AE3.
• The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
• Recently, a new member of AE family, AE4, has been cloned from rabbit and human.
• Human AE4 (945aa; chromosome 5) is predicted to have 14 TM domains.
• It is strongly expressed in the kidney.
• Rabbit and human AE4 share 84% identity.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-50,000.
• Coat ELISA plates at 1ug/ml with A2295-14G2-Anion Exchanger 4, Control Peptide Human
• Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.