Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport.
AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification.
AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3).
Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues.
AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin.
The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1.
In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3.
The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself
Human AE3 gene (chromosome 2q36) encodes a protein of 1233 aa (mouse/rat 1227 aa).
AE3 is primarily expressed in excitable tissue such cardiac muscle, retina, and brain.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml using the control peptide at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:A2295-14F2: Anion Exchanger 3, Rat Control Peptide
Storage and StabilityMay be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a powder lyophilized from a solution containing PBS, pH 7.4, and 0.1% BSA. Reconstitute wih 100ul sterile PBS.
Specificity：Recognizes rat Anion Exchanger 3. No significant sequence homology is detected with other AE or other proteins. Does not recognize cardiac AE3.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to aa13-32 near the N-terminal, cytoplasmic domain of rat Anion Exchanger 3 conjugated to KLH. Species Sequence Homology: mouse, 100%; human, 95%; rabbit, 90%.
