84-1040-60　Anion Exchanger 3 (AE3, Anion exchange protein 3, CAE3/BAE3, Cardiac/brain band 3-like protein, Neuronal band 3-like protein, SLC2C, Solute carrier family 4 member 3) 100ul　A2295-14F

特徴

• Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport.
• AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification.
• AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3).
• Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues.
• AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin.
• The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1.
• In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3.
• The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
• Human AE3 gene (chromosome 2q36) encodes a protein of 1233aa (mouse/rat 1227aa).
• AE3 is primarily expressed in excitable tissue such cardiac muscle, retina, and brain.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:5000 (ECL)ELISA: 1:10,000-1:50,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.