84-1040-56　Angiotensin Converting Enzyme 2 (ACE-2, ACEH) 100ug　A2295-01R

特徴

• Renin-Angiotensin System (RAS) is a crtical regulator of blood pressure homeostasis.
• The protease renin cleaves angiotensinogen into inactive decameric peptide angiotensin-I (Ang-I).
• Angiotensin-converting enzyme (ACE) then cleaves C-terminal dipeptide from Ang-I to form an active octamer angiotensin-II (Ang-II), which can contribute to hypertension by promoting vascular smooth muscle vasocontriction and renal tubule sodium reabsorption.
• ACE can also cleave many other small peptides including the vasodialating peptide bradykinin into inactive fragment, cleave Alzheimer amyloid beta-peptide (Abeta), retard Abeta aggregation, deposition and fibril formation.
• ACE mutant mice display spontaneous hypotension, partial male infertility and kidney malformations.
• ACE is found in somatic (s-ACE) and testicular/germinal (t-ACE) isoforms.
• The products of renin and ACE catalysis, namely Ang1-10 and Ang1-8 can also be by another peptidase, ACE-2 to Ang1-9 and Ang1-7, respectively.
• ACE-2 and ACE (s-ACE and t-ACE) are made as transmembrane (TM) proteins but these enzymes also exist as soluble, truncated forms lacking the TM and cytosolic domains.
• ACE-2 (also known as ACE-2 and ACE homolog, ACEH) gene has been mapped at human chromosome Xp22.
• ACE-2 enzymes from human (805aa) and mouse (798aa) are single chain proteins with 40% seq homology to N- and C-terminal domains of ACE.
• However, in contrast to s-ACE which consists of two catalytic sites, ACE-2 contains only one active site.
• Unlike s-ACE and t-ACE which are dipeptidyl-carboxypeptidases, ACE-2 acts as a carboxypeptidase, cleaving single residue from Ang-I, generating Ang1-9 and a single residue from Ang-II to generate Ang1-7.
• ACE-2 can cleave angiotensin-I but not bradykinin and the enzyme activity is not inhibited by the ACE inhibitors.
• This enzyme is expressed highly in heart, kidney and testis and moderately in colon, small intestine and ovary.
• ACE-2 is an essential regulator of heart fuction because targeted disruption of this enzyme in mice results in severe cardiac contractility defect, increased angiotensin-II levels and upregulation of hypoxia-induced genes in the heart.
• Human ACE-2 (1-740aa, glycosylated secreted protein of MW ~120kD, predicted MW 85kD) with His tag was expressed in mouse myeloma cell line NS0 and purified >90% (SDS-PGE).
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml.
• Recognize ~120kD ACE-2 under reducing and non-reducing conditions
• ELISA: 0.5-1ug/ml.
• Coat ELISA plates at 1ug/ml Control Peptide.
• Optimal dilutions to be determined by the researcher.
• Control Peptide: A2295-01J1 and A2295-01S.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.