84-1040-51　Angiotensin Converting Enzyme 2 (ACE-2, ACEH) 100ug　A2295-01H

特徴

• Renin-Angiotensin System (RAS) is a crtical regulator of blood pressure homeostasis.
• The protease renin cleaves angiotensinogen into inactive decameric peptide angiotensin-I (Ang-I).
• Angiotensin-converting enzyme (ACE) then cleaves C-terminal dipeptide from Ang-I to form an active octamer angiotensin-II (Ang-II), which can contribute to hypertension by promoting vascular smooth muscle vasocontriction and renal tubule sodium reabsorption.
• ACE can also cleave many other small peptides including the vasodialating peptide bradykinin into inactive fragment, cleave Alzheimer amyloid beta-peptide (Abeta), retard Abeta aggregation, deposition and fibril formation.
• ACE mutant mice display spontaneous hypotension, partial male infertility and kidney malformations.
• ACE is found in somatic (s-ACE) and testicular/germinal (t-ACE) isoforms.
• The products of renin and ACE catalysis, namely Ang1-10 and Ang1-8 can also be by another peptidase, ACE-2 to Ang1-9 and Ang1-7, respectively.
• ACE-2 and ACE (s-ACE and t-ACE) are made as transmembrane (TM) proteins.
• These enzymes also exist as soluble, truncated forms lacking the TM and cytosolic domains.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:Western Blot (Reducing and Non-reducing): 1-10ug/ml (ECL)ELISA: 0.5-1ug/ml.
• Coat ELISA plates at 1ug/ml with Control Peptide
• Optimal dilutions to be determined by the researcher.
• Control Peptide:A2295-01J: Angiotensin Converting Enzyme 2, Mouse (ACE-2, ACEH)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.