84-1040-50　Angiotensin II Type 1 Receptor (AT1) 100ug　A2295-08D

特徴

• Angiotensin II mediates its action by interacting with membrane receptor, Angiotensin II type 1 receptors.
• The signal is transmitted via G-proteins that activates a phosphatidylinositol- calcium second messenger system.
• ATII receptors are integral membrane proteins of approx.359-363aa and predicted to contain at least 7 transmembrane domains.
• The N and C-terminus are predicted to extracellular and cytoplasmic, respectively.
• Three isoforms of ATII receptors have been cloned.
• In rat type 1a (359 aa) is expressed in the liver kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscles (1).
• Rat Type 1b (also known as AT3; 359 aa) is found in liver, kidney, and aorta (2).
• Type 1c (177 aa) has been detected in brain, kidney, liver, spleen and lung (3).
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
• Detects ~45-kD band
• ELISA: 1:10,000-1:100,000 using 50-100ng of A2295-08K control peptide/well
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.