84-1040-43　Angiotensin II Type 1 Receptor (AT1) 100ul　A2295-07D

特徴

• Angiotensin II interacts with two types of G-protein coupled membrane receptors, AT1 (type 1) and AT2 (type 2).
• Major cardiovascular effects appear to be mediated through AT1.
• AT1 has three isoforms (rat AT1A 359aa; AT1B/AT III, 359aa; and AT1C, 177aa).
• Rat AT1 receptors are predicted to contain seven transmembrane domains.
• The N-terminus is predicted to be extracellular.
• The C-terminus is inside the membrane (cytoplasmic).
• The binding of angiotensin II with AT1 receptors activate a phosphatidylinositol-calcium second messenger system.
• AT1 receptors are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle.
• AT2 gene ( chromosome x) encodes 363 aa protein.
• It is highly expressed in myometrium with lower levels in adrenal and fallopian tube.
• It is also expressed at high levels in fetal kidney and intestine.
• Rat AT1 receptors are predicted to contain seven transmembrane domains.
• The N-terminus is predicted to be extracellular, while C-terminus inside the membrane (cytoplasmic).
• The binding of angiotensin II with AT1 receptors activate a phosphatidylinositol-calcium second messenger system.
• AT1 receptors are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle.
• AT2 gene (chromosome x) encodes 363aa protein.
• It is expressed at high levels in myometrium, fetal kidney and intestine.
• It is expressed at lower levels in adrenal and fallopian tube.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 (ECL).
• Detected ~45kD band
• ELISA: 1:10,000-1:100,000 using 50-100ng of A2295-07G Control peptide/well
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.