Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly.
One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 aa peptides (beta amyloid1-40,1-42, and 1-43; approx.
4.5kD).
Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein.
APP gene has 19 exons (1).
At least 8 different types of APP (APP695, APP751, and APP770 are the major ones) are produced as a result of alternative splicing of exons 7, 8, and 15.
Exons 7 codes for a 57-aa domain that is analogous to Kunitz protease inhibitors (KPI).
Exon 8 codes for a 19-aa residue domain with homology to the MRC OX-2 antigen found on the membrane of neurons, while exon 15 codes for an 18-aa domain, 16 aa proximal to the Ab region.
APP695 lacks exons 7 and 8, whereas APP770 and APP751 represents the full length and the exon 8 lacking isoform.
Beta amyloid A4 protein represents 597-638 aa of APP695 (28 aa from the extracellular domain and 14-15 aa from the extracellular domain).
APP695 is predominantly expressed in the brain while APP751 and APP770 are also found in other tissues
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500 using ECL
ELISA: 1:5000-1:50,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide, 40% glycerol.
Specificity：The human peptide sequence is found in all major APP isoforms (APP695, APP751 and APP770). It is 95% identical to porcine, mouse and rat, 90% with chicken APPs. Species crossreactivity has not been established.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A synthetic 20-aa peptide near the extracellular, N-terminus (44-63 aa) of human APP695 (1). Peptide was conjugated to KLH.
