The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1, and CGRP2) and adrenomedullin (ADM).
Calcitonin is 32 aa peptide found in the parafollicular "C" cells of the thyroid in mammals.
It is also found in a number of non-mammals.
It regulates the mineral (calcium and phosphate) balance.
Calcitonin causes hypercalcemia by acting as an inhibitor of osteoclast induced bone resorption.
CGRP is a 37-aa peptide produced by tissue specific processing of the calcitonin gene.
Calcitonin is the major product in the thyroid, whereas CGRP is the major product in neural tissues.
CGRP is a potent cardiovascular agent.
It has structural similarity with amylin.
CGRP is found in two isoforms (CGRP-I and CGRP-II) that differs only by 3 amino acids.
Amylin is a 37-aa peptide produced in the pancreatic beta-cell secretory granules and is co-released with insulin.
Amylin also has CGRP-like effects on bone metabolism.
Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism.
Adrenomedullin (ADM) is a 52-aa hypotensive peptide.
It has structural similarity with CGRP and amylin.
ADM is produced in peripheral tissues, adrenal medulla, lung, and kidney.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control Peptide:A2275-53D: Amylin, Human
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity：Recognizes human amylin.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic amino acid peptide mapping to amino acids 18-30 (HSSNNFGAILSST) of human IAPP, UniProt accession #P10997.
