The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1, and CGRP2) and adrenomedullin (ADM).
Amylin is a 37-aa peptide produced in the pancreatic beta- cell secretory granules and is co-released with insulin.
Amylin also has CGRP-like effects on bone metabolism.
Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism.
The calcitonin family peptides probably act through G-protein coupled membrane receptors.
Recently, a homolog of calcitonin receptor, CRLR (calcitonin- receptor-like receptor human 461 aa; rat/mouse 463 aa) was identified.
It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-50,000.
Coat ELISA plates at 1ug/ml with A2275-53D Amylin, Human (Control Peptide)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.09% sodium azide. Reconstitute with 100ul of sterile PBS.
Specificity：Recognizes human amylin. No significant sequence homology with other calcitonin family of peptides.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic amino acid peptide mapping to HSSNNFGAILSST, aa18-30, of human Amylin, conjugated to KLH.
