84-1040-24　Akt3 (RAC-PK Gamma) 100ul　A1125-32

特徴

• Putative human homologs of the protooncogene v-akt of the acutely transforming retrovirus AKT8 have been cloned.
• These protein-serine/threonine kinase proteins have a catalytic domain closely related to both PKA and PKC and have been designated rac (related to A and C kinases), pkb (Protein kinase B) or Akt.
• RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus.
• The Amino terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins.
• The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides.
• The kinase domain is located between residues 148 to 411.
• These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells.
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECLELISA:1:10,000-1:100,000 using 50-100ng of control peptide/well.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.