Putative human homologs of the protooncogene v-akt of the acutely transforming retrovirus AKT8 have been cloned.
These protein-serine/threonine kinase proteins have a catalytic domain closely related to both PKA and PKC and have been designated rac (related to A and C kinases), pkb (Protein kinase B) or Akt.
RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus.
The Amino terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins.
The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides.
The kinase domain is located between residues 148 to 411.
These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells.
AKT2 (RAC-PK-b;) has been shown to be amplified and over-expressed in some human carcinoma cell lines and primary tumors suggesting that it may contribute to the development of common epithelial tumors of the ovary (3).
It has been reported that anti-sense AKT2 can greatly inhibit the expression of AKT2 protein and suppress the tumorogenic phenotype of PANC1 cells inoculated s.c.
in nude mice.
This effect was restricted to cells that over-express AKT2.
It is postulated that over-expression of AKT2 could upregulate the mediation of growth signals that may contribute to cell proliferation.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL
ELISA:1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The rat control peptide is 93% conserved in mouse and human AKT2. No significant homology is seen with AKT1 or AKT3 or other kinases. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：A 16-aa peptide near the C-terminus of rat AKT2 (rat/mosue/human 481 aa) (1) was synthesized with an added N-terminal cysteine, conjugated to KLH
