84-1040-18　Agouti Protein 100ul　A1059-59B

特徴

• Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity.
• An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity.
• High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity.
• In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub).
• More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned.
• The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti.
• Agouti protein (132aa in human) is normally expressed in skin.
• Its ubiquitous expression, such as in rare allele lethal yellow, causes yellow coat., linear growth, immune defects and obesity.
• Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1 and possibly MC2R and MC4R).
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/per well
• Optimal dilutions to be determined by researcher.
• Control Peptide:A1059-58A: Agouti (Control Peptide)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.