84-1040-13　Adipsin 100ug　A0890

特徴

• Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides.
• Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need.
• The switch from energy storage to expenditure is finely regulated by a variety of hormones.
• In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis.
• Many of these genes are finely regulated during adipocyte differentiation and maturation.
• Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology.
• Adipsin is a serine protease that is secreted by adipocytes.
• It is deficient in several animal model of obesity.
• Adipsin has now been identified as the same protein as complement factor D.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:Western Blot:1-5 ug/ml using ECL.
• Adipsin is ~22 kDELISA: 1:10,000-50,000.
• Coat 10-100ng of A0890-01 control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide:A0890-02: Adipsin, Human (Control Protein for Western Blot)A0890-01: Adipsin (Blocking Peptide, ELISA Standard)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.