The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis.
Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs).
Based upon biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b and A3.
The high affinity receptor A1 inhibits adenyl cyclase via G proteins.
A2a receptor is believed to cause vasorelaxation in the coronary artery.
Various ARs and their subtypes have recently been cloned from several species.
ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains.
The N-termini and C-termini are predicted to be extracellular and cytoplasmic, respectively.
There is an overall 30% homology among the four ARs.
ARs are distributed throughout the body.
Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromosome 1).
Based upon mRNA studies, the A3R has been found in rat testis and lung, kidneys heart and brain (1).
Other studies have found the mRNA to be highest in lung, spleen, and pineal glands, and to a moderate levels in brain, kidney, and testis (2).
It has been found in lung, liver, kidney, and heart and very little in skeletal muscle and brain (3).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:10,000-50,000 using 50-100ng A3R control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: A0869-70G
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes Adenosine A3 Receptor. Species crossreactivity: The peptide used for making antibodies to rat A3R shows 46% homology (7/15 AA) with human and 20% (3/15 AA) with sheep A3R sequences. Antibody crossreactivity in various species is not established.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：A 15aa sequence corresponding to rat A3R gene. This region corresponds to the 3rd extracellular domain.
