The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis.
Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs).
Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b, and A3.
The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins.
A2a receptor is believed to cause vasorelaxation in coronary artery.
Various, ARs and their subtypes have recently been cloned from several species.
ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains.
The N-termini are predicted to be extracellular and the C-termini cytoplasmic.
There is an overall 30% homology among the four ARs.
ARs are distributed throughout the body.
Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL
ELISA: 1:10,000-50,000 using 50-100ng A1R control peptide/well
Immunohistochemistry (Frozen): 10-50ug/ml
Immunofluorescence: 10-50ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:A0869-66G: Adenosine A2b Receptor, Human (A2b) (Control Peptide)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity：Recognizes human Adenosine A2b Receptor. Species Crossreactivity: mouse and rat. Species Sequence Homology: rabbit, 87%; canine, 81%; rat, 75%, mouse, 62%
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A 16 aa sequence corresponding to human brain A2bR gene (1). This region corresponds to the 2nd extracellular domain. A cysteine has been added at the NH2 terminus, conjugated to KLH.
