84-1040-06　［取扱停止］Adenosine A2b Receptor (A2b) 100ul　A0869-66A

特徴

• The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis.
• Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs).
• Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b, and A3.
• The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins.
• A2a receptor is believed to cause vasorelaxation in coronary artery.
• Various, ARs and their subtypes have recently been cloned from several species.
• ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains.
• The N-termini are predicted to be extracellular and the C-termini cytoplasmic.
• There is an overall 30% homology among the four ARs.
• ARs are distributed throughout the body.
• Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1).
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL
• ELISA: 1:10,000-1:50,000 using 50-100ng A1R control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide: A0869-66G
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.