The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis.
Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs).
Based upon, biochemical and pharmacological criteria, AR s have been classified into A1, A2a, A2b , and A3 .
The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins.
A2a receptor is believed to cause vasorelaxation in coronary artery.
Various, ARs and their subtypes have recently been cloned from several species.
ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains.
The N-termini are predicted to be extracellular and the C-termini cytoplasmic.
There is an overall 30% homology among the four ARs.
ARs are distributed throughout the body.
Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:10,000-50,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Positive Control:A0869-56E
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes Adenosine A2a Receptor. Species crossreactivity: Human, bovine and porcine A2a receptors. The canine A2aR21 has homology of 43% (13/30 AA) with rat, 80% (24/30 AA) with human and 70% (21/30 AA) with G. pig A2aR sequences (1).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Bo Ca Hu Po Rt
Immunogen：A 30 amino acid sequence as corresponding to canine A2aR gene (1). This region is within the 4th C-terminal intracellular domain. A cysteine has been added at the NH2 terminus for coupling to KLH.
