The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis.
Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs).
Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b and A3.
The high affinity receptor A1 inhibits adenyl cyclase.
Low affinity receptor A2a stimulates the cyclase via G proteins.
A2a receptor is believed to cause vasorelaxation in coronary artery.
Various, ARs and their subtypes have recently been cloned from several species.
ARs belong to the superfamily of G-protein coupled receptors.
They are predicted to contain 7 transmembrane domains.
The N-termini are predicted to be extracellular.
The C-termini is predicted to be cytoplasmic.
There is an overall 30% homology among the four ARs.
ARs are distributed throughout the body.
Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL.
Detects 79 and 39kD bands in cortical membranes from rat, pig, and lamb.
The 79kD band can be converted to 39kD band by agonist or antagonist treatment and could represent a A1R dimers.
A1R, photoaffinity labeled with 125I-R-AHPIA as 39kD band
ELISA: 1:10,000-50,000 using 50-100ng A1R control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: A0869-52F
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes Adenosine A1 Receptor. Species crossreactivity: Rat, porcine, lamb. Species Sequence Homology: Mouse, canine, rabbit and human: 92%; bovine: 78%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide 14aa sequence corresponding to rat A1R gene. This region corresponds to the 3rd extracellular domain, N-terminus. A cysteine has been added at the NH2 terminus for coupling to KLH.
